LOCUS       UGT2B15_pGEc2-DE        6700 bp    DNA     circular     21-JAN-2013
FEATURES             Location/Qualifiers
     misc_feature    complement(6178..6307)
                     /note="3'-ITR"
     misc_feature    complement(5876..6090)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5328..5869
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4507..4529
                     /note="attB2"
     misc_feature    5307..5312
                     /note="BstB1 site"
     misc_feature    4530..5225
                     /note="Super GFP"
     misc_feature    5226..5276
                     /note="AviTag"
     misc_feature    5277..5300
                     /note="His Tag"
     PCR_frame       3002..4507
                     /note="Reading Frame"
                     /set="Set 1"
     source          3006..4484
                     /dnas_title="UGT2B15_pGEc2-DEST"
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="MGC:180134 IMAGE:100014811"
                     /clone_lib="NIH_MGC_428"
                     /lab_host="DH10B"
                     /focus
                     /note="Vector: pENTR223.1 with stop codon"
     source          3006..4484
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     gene            3006..4484
                     /dnas_title="UGT2B15"
                     /gene="UGT2B15"
                     /gene_synonym="UGT2B8"
                     /db_xref="GeneID:7366"
                     /db_xref="HGNC:12546"
                     /db_xref="MIM:600069"
     CDS             3006..4484
                     /dnas_title="UGT2B15"
                     /gene="UGT2B15"
                     /gene_synonym="UGT2B8"
                     /codon_start=1
                     /product="UDP glucuronosyltransferase 2 family,
                     polypeptide B15"
                     /protein_id="AAI46571.1"
                     /db_xref="GI:148922096"
                     /db_xref="GeneID:7366"
                     /db_xref="HGNC:12546"
                     /db_xref="MIM:600069"
                     /translation="MSLKWTSVFLLIQLSCYFSSGSCGKVLVWPTEYSHWINMKTILE
                     ELVQRGHEVTVLTSSASTLVNASKSSAIKLEVYPTSLTKNYLEDSLLKILDRWIYGVS
                     KNTFWSYFSQLQELCWEYYDYSNKLCKDAVLNKKLMMKLQESKFDVILADALNPCGEL
                     LAELFNIPFLYSLRFSVGYTFEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIHM
                     LYFDFWFQIYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVD
                     FVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQ
                     KVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMV
                     GIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPVYKENVMKLSRIH
                     HDQPMKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATVIFI
                     ITKFCLFCFRKLAKKGKKKKRD"
     misc_feature    3006..4484
                     /note="UGT2B15 coding region"
     misc_feature    4485..4505
                     /note="TEV site"
     PCR_primer      2992..3001
                     /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC"
                     /primer="CAGCTCTTCC"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
                     /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC"
     PCR_primer      complement(3002..4514)
                     /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG"
                     /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
                     /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG"
     PCR_product     complement(2992..4514)
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="1.000"
     vector          join(4515..6700,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4514
                     /source="UGT2B15-C-term-TEVfusion-pDONR221"
                     /type="Entry Clone"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCCatgtc tctgaaatgg acgtcagtct ttctgctgat acagctcagt tgttacttta
     3061 gctctggaag ctgtggaaag gtgctagtgt ggcccacaga atacagccat tggataaata
     3121 tgaagacaat cctggaagag cttgttcaga ggggtcatga ggtgactgtg ttgacatctt
     3181 cggcttctac tcttgtcaat gccagtaaat catctgctat taaattagaa gtttatccta
     3241 catctttaac taaaaattat ttggaagatt ctcttctgaa aattctcgat agatggatat
     3301 atggtgtttc aaaaaataca ttttggtcat atttttcaca attacaagaa ttgtgttggg
     3361 aatattatga ctacagtaac aagctctgta aagatgcagt tttgaataag aaacttatga
     3421 tgaaactaca agagtcaaag tttgatgtca ttctggcaga tgcccttaat ccctgtggtg
     3481 agctactggc tgaactattt aacataccct ttctgtacag tcttcgattc tctgttggct
     3541 acacatttga gaagaatggt ggaggatttc tgttccctcc ttcctatgta cctgttgtta
     3601 tgtcagaatt aagtgatcaa atgattttca tggagaggat aaaaaatatg atacatatgc
     3661 tttattttga cttttggttt caaatttatg atctgaagaa gtgggaccag ttttatagtg
     3721 aagttctagg aagacccact acattatttg agacaatggg gaaagctgaa atgtggctca
     3781 ttcgaaccta ttgggatttt gaatttcctc gcccattctt accaaatgtt gattttgttg
     3841 gaggacttca ctgtaaacca gccaaacccc tgcctaagga aatggaagag tttgtgcaga
     3901 gctctggaga aaatggtatt gtggtgtttt ctctggggtc gatgatcagt aacatgtcag
     3961 aagaaagtgc caacatgatt gcatcagccc ttgcccagat cccacaaaag gttctatgga
     4021 gatttgatgg caagaagcca aatactttag gttccaatac tcgactgtac aagtggttac
     4081 cccagaatga ccttcttggt catcccaaaa ccaaagcttt tataactcat ggtggaacca
     4141 atggcatcta tgaggcgatc taccatggga tccctatggt gggcattccc ttgtttgcgg
     4201 atcaacatga taacattgct cacatgaaag ccaagggagc agccctcagt gtggacatca
     4261 ggaccatgtc aagtagagat ttgctcaatg cattgaagtc agtcattaat gaccctgtct
     4321 ataaagagaa tgtcatgaaa ttatcaagaa ttcatcatga ccaaccaatg aagcccctgg
     4381 atcgagcagt cttctggatt gagtttgtca tgcgccacaa aggagccaag caccttcgag
     4441 tcgcagctca caacctcacc tggatccagt accactcttt ggatgaaaac ttgtactttc
     4501 aaggctCCAG CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG CGAGGAACTG TTCACCGGCG
     4561 TGGTGCCCAT CCTGGTGGAA CTGGATGGCG ACGTGAACGG CCACAAGTTC TCTGTGCGGG
     4621 GAGAGGGCGA AGGCGACGCC ACAAATGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG
     4681 GCAAGCTGCC CGTGCCTTGG CCTACCCTCG TGACCACACT GACCTACGGC GTGCAGTGCT
     4741 TCAGCAGATA CCCCGACCAT ATGAAGCGGC ACGACTTCTT CAAGAGCGCC ATGCCCGAGG
     4801 GCTACGTGCA GGAACGGACC ATCAGCTTCA AGGACGACGG CACCTACAAG ACCAGAGCCG
     4861 AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA ACCGGATCGA GCTGAAGGGC ATCGATTTCA
     4921 AAGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTGT
     4981 ACATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACG
     5041 TGGAAGATGG CAGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGAGATG
     5101 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GAGCGTGCTG AGCAAGGACC
     5161 CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTCGT GACCGCCGCT GGCATCACCC
     5221 ACGGCATGAG CGGCCTGAAC GACATCTTCG AGGCCCAGAA AATCGAGTGG CACGAGCACC
     5281 ACCATCATCA CCATCACCAC TGATGATTCG AAAAATAATA AGGATCCAAT CAACCTCTGG
     5341 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
     5401 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
     5461 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA
     5521 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG
     5581 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG
     5641 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA
     5701 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA
     5761 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC
     5821 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC
     5881 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC
     5941 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG
     6001 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA
     6061 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA
     6121 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG
     6181 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG
     6241 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG
     6301 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC
     6361 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT
     6421 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT
     6481 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
     6541 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT
     6601 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT
     6661 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG
//

